The production process of xanthus sorbifolium protein
The kernels of X. sorbifolium contain both oil and protein. The kernels contain 58.91% oil, 14 kinds of fatty acids, and the content of unsaturated fatty acids is as high as 94%. The kernels contain 25.75% protein, 18 kinds of amino acids, and the types of essential amino acids Complete, and the amino acid score is high, with high nutritional value.
The extraction methods of plant protein mainly include water extraction, alkali-soluble acid precipitation method, organic solvent extraction method, membrane separation method, etc. Among them, the alkali-soluble acid precipitation method has become a classic because of its simplicity, rapid separation, and stable quality of the obtained product. The protein extraction method is especially suitable for the extraction of protein from fruits, seeds and other crops. When using the pressing method to produce Xanthos sorbifolium seed oil, the obtained oil residue contains a large amount of protein, starch and woody components of the seed coat, which has high comprehensive development and utilization value and can be used for high-protein food and feed. Some studies have analyzed the amino acid composition of X. sorbifolia protein, investigated and optimized the process conditions for extracting and separating protein from X. Comprehensive development and utilization provide technical reference.
Weigh a certain amount of Xantho sorbifolium oil residue sample, add the buffer extract with corresponding pH according to a certain material-to-liquid ratio, leaching in a water bath with magnetic stirring at a certain temperature for a certain period of time, then centrifuge at 5000r/min for 15min at room temperature, discard After deprecipitation, the supernatant was appropriately diluted and stained with Coomassie Brilliant Blue G-250 to measure the protein content and calculate the protein yield. When carrying out acid precipitation separation, the protein extract obtained by the above method is adjusted to different pHs with 1mol/L citric acid to carry out sufficient sedimentation, after the sedimentation is complete, centrifuge at 5000r/min for 15min, take the supernatant and settle to 50mL and then measure the protein content , to calculate the protein sedimentation rate; the precipitate obtained by centrifugation was washed with water until neutral, and the protein purity was determined after drying.
Through single factor experiment and orthogonal experiment optimization, the optimal process conditions for the extraction of X. sorbifolium protein by alkali-soluble acid precipitation method were: solid-liquid ratio 1:30, extraction pH 9, extraction time 150 min, and extraction temperature 60 °C. Extracted twice under these conditions, the protein yield was 22.58%; the optimal pH for citric acid precipitation to separate X. sorbifolium protein was 4.6, and the protein sedimentation rate was 94.67%. Alkali-dissolving and acid-precipitating method can increase the purity of X. sorbifolium protein from 38.46% to 84.03%, and the purification factor is 2.18 times.
